Journal: International Journal of Pharmaceutics: X
Article Title: Ensuring vaccine cold chain integrity: A rapid and low-cost test for identifying heat-exposed sucrose-containing vaccines
doi: 10.1016/j.ijpx.2025.100467
Figure Lengend Snippet: A colorimetric glucose assay was used to measure levels of glucose in 30 μg adult and 10 μg children's COMIRNATY™ vaccines after exposure to different temperature conditions. (A) Photograph of the plate showing an increase in glucose at 37 °C and a further increase at 45 °C. The difference in colour intensity for 37 °C and 45 °C compared to 2–8 °C storage can be observed by eye without the need for a plate reader. (B) The intensity of the colour change was also analysed using a spectrophotometer at 540 nm and the concentrations of glucose in the vaccine samples were determined based on the glucose standards. The levels of glucose in temperature-altered conditions were compared to the recommended 2–8 °C storage temperature. Error bars show the standard deviations from three measurements for each temperature point. 3× FT, three freeze-thaw cycles; RT, room temperature. Ordinary one-way ANOVA with Dunnett's multiple comparisons tests. ns, not significant; ****p < 0.001.
Article Snippet: The glucose concentrations in COMIRNATYTM vaccine samples were measured using a colorimetric glucose assay kit (Cell Biolabs, San Diego, CA, USA), according to the manufacturer's protocol.
Techniques: Glucose Assay, Vaccines, Spectrophotometry